human calabrese crispr activation pooled library set a11  (Addgene inc)

Journal: bioRxiv

Article Title: A whole genomic CRISPR-Cas9 screen identifies the amino acid transporter SLC43A1 (LAT3) as a major determinant of oxaliplatin sensitivity in colorectal cancer cells

doi: 10.1101/2025.04.21.649594

Figure Lengend Snippet: A) Overview of CRISPR screen methodology (adapted from ). B) SW620 KO screen;-log 10 RRA scores in cells treated with 0.38µM ( left ) or 0.5µM OxPt ( right ) at Day 20 compared to control untreated cells at Day 20. C) RKO KO screen;-log 10 RRA scores in cells treated with 1.9µM OxPt at Day 20 compared to control untreated cells at Day 20. D) SW620 CRISPRa screen;-log 10 RRA scores in cells treated with 0.38µM ( left ) or 0.5µM OxPt ( right ) at Day 20 compared to control untreated cells at Day 20. E) RKO CRISPRa screen;-log 10 RRA scores in cells treated with 1.9µM at Day 20 compared to control untreated cells at Day 20. In each graph, top 5 hits are labeled; significant hits are colored (blue or lilac) while non-significant hits are in grey.

Article Snippet: The Human Calabrese CRISPR Activation Pooled Library Set A was a gift from David Root and John Doench (Addgene viral prep #92379-LVC) ( ).

Techniques: CRISPR, Control, Labeling

Journal: Cancer Discovery

Article Title: Paradoxical Activation of Oncogenic Signaling as a Cancer Treatment Strategy

doi: 10.1158/2159-8290.CD-23-0216

Figure Lengend Snippet: LB-100 activates oncogenic signaling, engages stress response pathways, and restrains the proliferation of colorectal cancer cells. A , Gene set enrichment analyses on time-course transcriptome data from HT-29 and SW-480 cells show selected “Hallmarks” and “KEGG” molecular signatures modulated by LB-100 (4 μmol/L). Darker bars indicate time points for which the respective gene set was significantly enriched (P <0.05). B , Time-course western blots show selected oncogenic signaling and stress response pathways modulated by LB-100 (4 μmol/L) in HT-29 and SW-480 cells. α-Tubulin and Vinculin were used as loading controls. C , IncuCyte-based proliferation assays with the colorectal cancer models in the absence or presence of LB-100 at 1, 2, or 4 μmol/L for the indicated times. D , String network combining all hits identified by the two independent genome-wide CRISPR screens (Supplementary Fig. S2) as modulators of LB-100 toxicity. Only high-confidence interactions are shown and disconnected nodes are omitted. Green nodes: CRISPRa screen; orange nodes: CRISPR-KO screen; yellow node: identified on both screens. E , GO analyses using the full list of hits from both CRISPR screens (Supplementary Fig. S2) as input. The top 5 enriched Biological Processes and Molecular Functions terms are shown. Darker bars highlight WNT/β-catenin-and MAPK-related terms.

Article Snippet: HT29 dCas9-VP64 clone E cells were transduced with lentivirus of Calabrese pooled human CRISPRa library sets A and B (Addgene, 92379 and 92380) separately, in the presence of 8 μg/mL polybrene (Santa Cruz, sc-134220A), and at an MOI of approximately 0.3.

Techniques: Western Blot, Genome Wide, CRISPR